Nucleocapsid, nuclear association, and genome location of the herpes simplex virus type 2 38-kD DNA-binding protein

Intervirology. 1990;31(2-4):76-84. doi: 10.1159/000150141.

Abstract

A previously-described herpes simplex virus type 2 DNA-binding protein with a molecular size of 38 kD has been further characterized. Using purified nucleocapsids, a DNase release assay, and intertypic recombinants, this protein was found to be a component of the nucleocapsid, intimately associated with the nuclear matrix, and encoded between 0.605 and 0.720 on the herpes simplex virus type 2 genome.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Capsid / analysis*
  • Cells, Cultured
  • Cricetinae
  • DNA-Binding Proteins / analysis*
  • DNA-Binding Proteins / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Viral
  • Immunoblotting
  • Nuclear Matrix / microbiology
  • Plasmids / genetics
  • RNA, Messenger / genetics
  • Simplexvirus / analysis*
  • Vero Cells
  • Viral Core Proteins / analysis*
  • Viral Proteins / analysis*
  • Viral Proteins / genetics

Substances

  • DNA-Binding Proteins
  • RNA, Messenger
  • Viral Core Proteins
  • Viral Proteins