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Nat Methods. 2011 Jun 5;8(7):568-70. doi: 10.1038/nmeth.1622.

Near-infrared branding efficiently correlates light and electron microscopy.

Author information

1
Department of Physiology, Indiana University School of Medicine-Muncie, Muncie, Indiana, USA.

Abstract

The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.

PMID:
21642966
DOI:
10.1038/nmeth.1622
[Indexed for MEDLINE]

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