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Nat Methods. 2011 Jun 5;8(7):592-8. doi: 10.1038/nmeth.1625.

High-throughput behavioral analysis in C. elegans.

Author information

1
Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.

Abstract

We designed a real-time computer vision system, the Multi-Worm Tracker (MWT), which can simultaneously quantify the behavior of dozens of Caenorhabditis elegans on a Petri plate at video rates. We examined three traditional behavioral paradigms using this system: spontaneous movement on food, where the behavior changes over tens of minutes; chemotaxis, where turning events must be detected accurately to determine strategy; and habituation of response to tap, where the response is stochastic and changes over time. In each case, manual analysis or automated single-worm tracking would be tedious and time-consuming, but the MWT system allowed rapid quantification of behavior with minimal human effort. Thus, this system will enable large-scale forward and reverse genetic screens for complex behaviors.

PMID:
21642964
PMCID:
PMC3128206
DOI:
10.1038/nmeth.1625
[Indexed for MEDLINE]
Free PMC Article

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