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Nat Methods. 2011 Jun 5;8(7):571-3. doi: 10.1038/nmeth.1624.

Sharper low-power STED nanoscopy by time gating.

Author information

1
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany.

Abstract

Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

PMID:
21642963
DOI:
10.1038/nmeth.1624
[Indexed for MEDLINE]

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