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ACS Comb Sci. 2011 Jul 11;13(4):347-50. doi: 10.1021/co2000373. Epub 2011 Jun 3.

High-throughput screening of small molecule libraries using SAMDI mass spectrometry.

Abstract

High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome fluorescent reporters or antibodies and often produce false-positive hits. The development of "label-free" assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery. This paper describes a high-throughput, label-free assay that combines self-assembled monolayers with mass spectrometry, in a technique called SAMDI, as a tool for screening libraries of 100,000 compounds in one day. This method is fast, has high discrimination, and is amenable to a broad range of chemical and biological applications.

PMID:
21639106
PMCID:
PMC3132997
DOI:
10.1021/co2000373
[Indexed for MEDLINE]
Free PMC Article

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