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PLoS One. 2011;6(5):e19254. doi: 10.1371/journal.pone.0019254. Epub 2011 May 26.

Choosing and using a plant DNA barcode.

Author information

1
Genetics and Conservation Section, Royal Botanic Garden Edinburgh, Edinburgh, United Kingdom. P.Hollingsworth@rbge.org.uk

Abstract

The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. This approach was successfully pioneered in animals using a portion of the cytochrome oxidase 1 (CO1) mitochondrial gene. In plants, establishing a standardized DNA barcoding system has been more challenging. In this paper, we review the process of selecting and refining a plant barcode; evaluate the factors which influence the discriminatory power of the approach; describe some early applications of plant barcoding and summarise major emerging projects; and outline tool development that will be necessary for plant DNA barcoding to advance.

PMID:
21637336
PMCID:
PMC3102656
DOI:
10.1371/journal.pone.0019254
[Indexed for MEDLINE]
Free PMC Article

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