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Nat Protoc. 2011 Jun;6(6):859-69. doi: 10.1038/nprot.2011.333. Epub 2011 May 26.

Monitoring protein expression in whole-cell extracts by targeted label- and standard-free LC-MS/MS.

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1
Mass Spectrometry Unit, Max Planck Institute for Molecular Genetics, Berlin, Germany.

Abstract

Targeted quantification of proteins is a daily task in biological research but often relies on techniques such as western blotting that are only barely quantitative. Here we present a broadly applicable workflow for protein quantification from unpurified whole-cell extracts that can be completed in less than 3 d. Without prefractionation or affinity enrichment, a whole-cell extract is trypsin-digested in an acetonitrile-containing ammonium carbonate buffer and high-molecular-weight compounds are removed by filtration. A normalization strategy, which involves endogenous reference proteins, facilitates the determination of relative changes in protein expression without requiring isotope labeling or standard addition. On a triple-quadrupole mass spectrometer, we demonstrate standard-free quantification of yeast proteins present over five orders of magnitude and present at ≥500 copies per cell. Liquid chromatography/multiple reaction monitoring (LC-MRM)-based proteomics is therefore a next-generation alternative to western blotting, as it allows simultaneous and reliable quantification of multiple endogenous proteins without the need for enrichment, isotope labeling or use of antibodies.

PMID:
21637204
DOI:
10.1038/nprot.2011.333
[Indexed for MEDLINE]
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