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J Vis Exp. 2011 May 21;(51). pii: 2692. doi: 10.3791/2692.

Creation of reversible cholestatic rat model.

Author information

1
Department of General Surgery, Providence Hospital and Medical Centers, RI, USA.

Abstract

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and affect the nutritional status and surgical outcomes. Study of the pathophysiological changes occurring in the liver and other organs is of importance. Various studies have been done in cholestatic rat models. We used a reversible cholestatic rat model in our recent study looking at the role of methylprednisolone in the ischemia reperfusion injury. Various techniques for creation of a reversible cholestatic model have been described. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model. This model can be used in various studies, such as looking at the changes in nutritional, physiological, pathological, histological and immunological changes in the gastrointestinal tract. This model can also be used to see the effects of cholestasis and various therapeutic interventions on major hepatic surgeries.

PMID:
21633335
PMCID:
PMC3197111
DOI:
10.3791/2692
[Indexed for MEDLINE]
Free PMC Article

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