Format

Send to

Choose Destination
Genesis. 2011 Jun;49(6):472-8. doi: 10.1002/dvg.20737. Epub 2011 May 31.

Generation of transgenic dogs that conditionally express green fluorescent protein.

Author information

1
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 151-744, Korea.

Abstract

We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.

PMID:
21630415
DOI:
10.1002/dvg.20737
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center