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PLoS Pathog. 2011 May;7(5):e1002046. doi: 10.1371/journal.ppat.1002046. Epub 2011 May 19.

Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.

Author information

1
Malaria Unit, Instituto de Medicina Molecular, Lisboa, Portugal.

Abstract

Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism.

PMID:
21625527
PMCID:
PMC3098293
DOI:
10.1371/journal.ppat.1002046
[Indexed for MEDLINE]
Free PMC Article

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