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Biochem Pharmacol. 2011 Aug 15;82(4):411-7. doi: 10.1016/j.bcp.2011.05.009. Epub 2011 May 18.

Analysis of deoxyribonucleotide pools in human cancer cell lines using a liquid chromatography coupled with tandem mass spectrometry technique.

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1
Department of Pharmacology, Section of Medical Oncology, Yale School of Medicine, New Haven, CT 06520, USA. wei.zhang.wz87@yale.edu

Abstract

Endogenous ribonucleotides and deoxyribonucleotides play a critical role in cell function, and determination of their levels is of fundamental importance in understanding key cellular processes involved in energy metabolism and molecular and biochemical signaling pathways. In this study, we determined the respective ribonucleotide and deoxyribonucleotide pool sizes in different human cell lines using a simple sample preparation method and LC/MS/MS. This assay was used to determine alterations in deoxyribonucleotide pools in human pancreatic PANC1 cells in response to hypoxia and to treatment with either hydroxyurea or aphidicolin. The levels of all deoxyribonucleotide metabolites decreased with hypoxia treatment, except for dUMP, which increased by two-fold. This LC/MS/MS assay is simple, fast, and sensitive, and it represents a significant advance over previously published methodologies.

PMID:
21620803
PMCID:
PMC3138996
DOI:
10.1016/j.bcp.2011.05.009
[Indexed for MEDLINE]
Free PMC Article
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