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Am J Bot. 2011 Apr;98(4):e96-9. doi: 10.3732/ajb.1000263. Epub 2011 Mar 30.

Development of new microsatellite markers from Mango (Mangifera indica) and cross-species amplification.

Author information

1
Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post,Bangalore 560 089, India. kv_ravishankar@yahoo.co.in

Abstract

PREMISE OF THE STUDY:

Microsatellite markers were developed and characterized to assess the genetic diversity among mango (Mangifera indica) cultivars and to test their amplification in closely related species.

METHODS AND RESULTS:

Thirty-six microsatellite (simple sequence repeats; SSR) loci were isolated by a microsatellite-enriched partial genomic library method. Primers designed for these loci were characterized using 30 diverse mango cultivars. The number of alleles ranged from 3 to 19 with an average of 9.2 alleles per locus. Polymorphic information content values ranged from 0.185 to 0.920 with a mean of 0.687. The total value for the probability of identity was 2.42 × 10(-31).

CONCLUSIONS:

The newly identified SSRs would be useful in genetic diversity studies, finger-printing, and mapping. Loci from five related species, M. odorata, M. anadamanica, M. zeylanica, M. camptosperma, and M. griffithii, were successfully amplified using these SSR primers, showing their potential utility across species.

PMID:
21613158
DOI:
10.3732/ajb.1000263
[Indexed for MEDLINE]
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