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Biochem Biophys Res Commun. 1990 May 16;168(3):979-86.

Characterization and tissue distribution of H3 histamine receptors in guinea pigs by N alpha-methylhistamine.

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Schering-Plough Research, Department of Allergy and Immunology, Bloomfield, New Jersey 07003.


We have used [3H]N alpha-methylhistamine to characterize H3-binding in the guinea pig brain and to study its tissue distribution. Kinetic and equilibrium binding experiments indicate a single class of high affinity sites in membranes isolated from guinea pig brain tissue (Kd = 0.4 nM, Bmax = 41 fmol/mg of protein). Competition binding experiments have confirmed that this ligand associates with H3-receptors and, under the conditions used in these experiments, does not bind to H1- or H2-receptors. Although there was some binding in the ileum and large intestine, H3-binding was found primarily in the central nervous system.

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