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Theranostics. 2011;1:277-89. Epub 2011 May 10.

Utilization of I-domain of LFA-1 to Target Drug and Marker Molecules to Leukocytes.

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1
1. Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047, USA.

Abstract

The long-term objective of this project is to utilize the I-domain protein for the α-subunit of LFA-1 to target drugs to lymphocytes by binding to ICAM receptors on the cell surface. The short-term goal is to provide proof-of-concept that I-domain conjugated to small molecules can still bind to and uptake by ICAM-1 on the surface of lymphocytes (i.e., Raji cells). To accomplish this goal, the I-domain protein was labeled with FITC at several lysine residues to produce the FITC-I-domain and CD spectroscopy showed that the FITC-I-domain has a secondary structure similar to that of the parent I-domain. The FITC-I-domain was taken up by Raji cells via receptor-mediated endocytosis and its uptake can be blocked by anti-I-domain mAb but not by its isotype control. Antibodies to ICAM-1 enhance the binding of I-domain to ICAM-1, suggesting it binds to ICAM-1 at different sites than the antibodies. The results indicate that fluorophore modification does not alter the binding and uptake properties of the I-domain protein. Thus, I-domain could be useful as a carrier of drug to target ICAM-1-expressing lymphocytes.

KEYWORDS:

FITC-I-domain; ICAM-1; Raji cells; binding; endocytosis

PMID:
21611107
PMCID:
PMC3100608
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