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Angew Chem Int Ed Engl. 2011 Jun 14;50(25):5618-28. doi: 10.1002/anie.201006896. Epub 2011 May 23.

In vivo solid-phase microextraction in metabolomics: opportunities for the direct investigation of biological systems.

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  • 1Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2 L 3G1, Canada.


Sample preparation has a strong impact on the quality of metabolomics studies. The use of solid-phase microextraction (SPME), particularly its in vivo format, enables the capture of a more representative metabolome and presents opportunities to detect low-abundance, short-lived, and/or unstable species not easily captured by traditional methods. The technique is ideally suited for temporal, spatial, and longitudinal studies of the same living system, as well as multicompartmental studies of the same organism. SPME is useful for the investigation of biological systems ranging in complexity from cells to mammalian tissues. Selected examples are highlighted in this Minireview in order to place the technique within the context of conventional methods of sample preparation for metabolomics.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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