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Methods Mol Biol. 2011;746:425-40. doi: 10.1007/978-1-61779-126-0_25.

Analysis of GPCR localization and trafficking.

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  • 1Department of Psychiatry, University of California, San Francisco, CA, USA.

Abstract

Localization and trafficking of G protein-coupled receptors (GPCRs) is increasingly recognized to play a fundamental role in receptor-mediated signaling and its regulation. Individual receptors, including closely homologous subtypes with otherwise similar functional properties, can differ considerably in their membrane trafficking properties. In this chapter, we describe several approaches for experimentally assessing the subcellular localization and trafficking of selected GPCRs. Firstly, we describe a flexible method for receptor localization using fluorescence microscopy. We then describe two complementary approaches, using fluorescence flow cytometry and surface biotinylation, for examining receptor internalization and trafficking in the endocytic pathway.

PMID:
21607873
PMCID:
PMC4960980
DOI:
10.1007/978-1-61779-126-0_25
[PubMed - indexed for MEDLINE]
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