Format

Send to

Choose Destination
PLoS One. 2011;6(5):e19713. doi: 10.1371/journal.pone.0019713. Epub 2011 May 13.

One for all--a highly efficient and versatile method for fluorescent immunostaining in fish embryos.

Author information

1
Centre for Organismal Studies, University of Heidelberg, Heidelberg, Baden-Württemberg, Germany. daigo.inoue@cos.uni-heidelberg.de

Abstract

BACKGROUND:

For the detection and sub-cellular (co)-localization of proteins in the context of the tissue or organism immunostaining in whole mount preparations or on sections is still the best approach. So far, each antibody required its own fixation and antigen retrieval protocol so that optimizing immunostaining turned out to be tedious and time consuming.

METHODOLOGY/PRINCIPAL FINDING:

Here we present a novel method to efficiently retrieve the antigen in a widely applicable standard protocol, facilitating fluorescent immunostaining of both cryosections and whole mount preparations in zebrafish (Danio rerio) and medaka (Oryzias latipes).

CONCLUSIONS/SIGNIFICANCE:

Our method overcomes the loss of sections and damage of tissue and cell morphology, and allows parallel immunostaining in multiple colors, co-immunostaining with fluorescent proteins in transgenic fish lines and in combination with whole mount in situ hybridization.

PMID:
21603650
PMCID:
PMC3094454
DOI:
10.1371/journal.pone.0019713
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center