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J Proteomics. 2011 Sep 6;74(10):2210-3. doi: 10.1016/j.jprot.2011.04.029. Epub 2011 May 9.

Charge state-selective separation of peptides by reversible modification of amino groups and strong cation-exchange chromatography: evaluation in proteomic studies using peptide-centric database searches.

Author information

1
Center for Genetic Engineering and Biotechnology, Cubanacán, Habana, Cuba. lazaro.betancourt@cigb.edu.cu

Abstract

Here we describe an integrated approach for the selective separation of peptides from complex mixtures using strong cation-exchange chromatography. The procedure exploits the charge differences produced by reversible modification of primary amino groups in peptides, enabling their separation into three major fractions: 1) neutral peptides 2) peptides with one positive charge and 3) peptides with 2 or more positive charges. The procedure demonstrated an excellent selectivity which allowed restricted MS/MS ion searches with peptide-centric databases.

PMID:
21596166
DOI:
10.1016/j.jprot.2011.04.029
[Indexed for MEDLINE]

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