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Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10121-6. doi: 10.1073/pnas.1018532108. Epub 2011 May 18.

Ligand-gated diffusion across the bacterial outer membrane.

Author information

1
University of Massachusetts Medical School, Program in Molecular Medicine, 373 Plantation Street, Worcester, MA 01605, USA.

Abstract

Ligand-gated channels, in which a substrate transport pathway is formed as a result of the binding of a small-molecule chemical messenger, constitute a diverse class of membrane proteins with important functions in prokaryotic and eukaryotic organisms. Despite their widespread nature, no ligand-gated channels have yet been found within the outer membrane (OM) of Gram-negative bacteria. Here we show, using in vivo transport assays, intrinsic tryptophan fluorescence and X-ray crystallography, that high-affinity (submicromolar) substrate binding to the OM long-chain fatty acid transporter FadL from Escherichia coli causes conformational changes in the N terminus that open up a channel for substrate diffusion. The OM long-chain fatty acid transporter FadL from E. coli is a unique paradigm for OM diffusion-driven transport, in which ligand gating within a β-barrel membrane protein is a prerequisite for channel formation.

PMID:
21593406
PMCID:
PMC3121852
DOI:
10.1073/pnas.1018532108
[Indexed for MEDLINE]
Free PMC Article

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