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J Am Chem Soc. 2011 Jun 22;133(24):9370-5. doi: 10.1021/ja200128n. Epub 2011 May 27.

In vitro reconstitution of eukaryotic ion channels using droplet interface bilayers.

Author information

1
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom.

Abstract

The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.

PMID:
21591742
DOI:
10.1021/ja200128n
[Indexed for MEDLINE]

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