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Mitochondrion. 2012 Jan;12(1):120-5. doi: 10.1016/j.mito.2011.04.006. Epub 2011 May 8.

The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore.

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1
School of Biochemistry and The Bristol Heart Institute, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK.

Abstract

Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibits pore opening whether or not Pi is present. Using siRNA in HeLa cells we could reduce PiC expression by 65-80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening.

PMID:
21586347
PMCID:
PMC3281194
DOI:
10.1016/j.mito.2011.04.006
[Indexed for MEDLINE]
Free PMC Article
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