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Mol Ecol Resour. 2008 Nov;8(6):1457-9. doi: 10.1111/j.1755-0998.2008.02217.x. Epub 2008 Sep 15.

Eight new tetranucleotide microsatellite loci for the agile frog (Rana dalmatina).

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Max-Planck Institute for Evolutionary Biology, 24306 Plön, Germany, Unit of Evolutionary Genetics, Institute for Genetics, University of Cologne, 50674 Cologne, Germany, Institute of Zoology, University of Veterinary Medicine, 30559 Hannover, Germany, Group of Molecular Ecology and Behaviour, University of Bielefeld, 33615 Bielefeld, Germany.


We describe eight new polymorphic tetranucleotide microsatellite loci isolated from the agile frog (Rana dalmatina). In 25 individuals from the Nature Reserve Lüneburger Heide (Lower Saxony, Germany), the number of alleles per locus ranged from four to nine and average observed heterozygosities from 69.1% to 80.7%. No evidence for linkage disequilibrium was found and none of the loci showed significant deviation from Hardy-Weinberg expectations. These microsatellite DNA markers are suitable tools for addressing population genetics issues in this endangered species.

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