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Mol Ecol Resour. 2008 Nov;8(6):1285-7. doi: 10.1111/j.1755-0998.2008.02356.x.

Isolation and characterization of 10 highly polymorphic di- and trinucleotide microsatellite markers in the mayfly Ameletus inopinatus (Ephemeroptera: Siphlonuridae).

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Research Institute Senckenberg, Department of Limnology and Conservation, Clamecystrasse 12, 63571 Gelnhausen, Germany, Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605-2496, USA, Institute of Zoology, Department of Molecular Ecology, Johannes Gutenberg University, J.J. Becherweg 13, 55128 Mainz, Germany.


We describe the isolation of ten polymorphic microsatellite loci from the mayfly Ameletus inopinatus. Loci had di- or trinucleotide repeat motifs and were highly variable with three to 17 alleles (mean = 7.15). Observed heterozygosity ranged from 0.143 to 0.905. One locus (Ami_202) showed significant deviation from Hardy-Weinberg equilibrium in one population, but no evidence for null alleles. One locus (Ami_73) was significantly linked with three other loci. The remaining nine loci should prove highly informative for population genetic studies.

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