Isolation and characterization of microsatellite loci in Pitcairnia albiflos (Bromeliaceae), an endemic bromeliad from the Atlantic Rainforest, and cross-amplification in other species

G M Paggi; C Palma-Silva; F Bered; F W Cidade; A C B Sousa; A P Souza; T Wendt; C Lexer

doi:10.1111/j.1755-0998.2008.02126.x

Isolation and characterization of microsatellite loci in Pitcairnia albiflos (Bromeliaceae), an endemic bromeliad from the Atlantic Rainforest, and cross-amplification in other species

Mol Ecol Resour. 2008 Sep;8(5):980-2. doi: 10.1111/j.1755-0998.2008.02126.x. Epub 2008 Jun 28.

Authors

G M Paggi¹, C Palma-Silva, F Bered, F W Cidade, A C B Sousa, A P Souza, T Wendt, C Lexer

Affiliation

¹ Departamento de Genética, IB/UFRGS, 91501-970 Porto Alegre, RS, Brazil, Departamento de Genética e Evolução, IB/UNICAMP, 13083-970 Campinas, SP, Brazil, Centro de Biologia Molecular e Engenharia Genética (CBMEG), UNICAMP, 13083-970 Campinas, SP, Brazil, Departamento de Botânica, CCS/IB/UFRJ, 21941-590 Rio de Janeiro, RJ, Brazil, Jodrell Laboratory, Royal Botanic Gardens Kew, Richmond, Surrey TW9 3DS, UK.

PMID: 21585947
DOI: 10.1111/j.1755-0998.2008.02126.x

Abstract

Eight microsatellite markers were isolated from Pitcairnia albiflos, an endangered endemic bromeliad species restricted to 'inselberg' rock outcrops in the state of Rio de Janeiro, Brazil. The number of alleles observed for each locus ranged from two to 12. Average observed and expected heterozygosities were 0.408 and 0.663, respectively. The cross-amplification test in 16 taxa suggests that the markers will be useful in numerous related bromeliad species. The loci will be used to study genetic structure and reproductive biology in fragmented inselberg populations and the origin and maintenance of barriers to gene flow between sympatric Pitcairnia species.