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J Autom Methods Manag Chem. 2011;2011:143416. doi: 10.1155/2011/143416. Epub 2011 Mar 30.

Flow-injection preconcentration of chloramphenicol using molecularly imprinted polymer for HPLC determination in environmental samples.

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Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.


The residue of antibiotic chloramphenicol (CAP) is important issue for food quality control and also for the environmental monitoring. It is banned for use in food-producing animals and has very limited use in human medicine, because of its severe impact on human health. Determination of trace level of CAP in environmental samples requires a very sensitive analytical method and efficient preconcentration procedure. CAP can be efficiently preconcentrated in flow-injection system using flow-through reactor packed with molecularly imprinted polymer (MIP), but determination of CAP in eluate from MIP requires the application of chromatographic separation, which was made in reversed-phase HPLC system with UV detection. In optimized conditions the limit of detection for 100 mL sample in HPLC with offline preconcentration on MIP was evaluated as 0.66 mg/L. In hyphenated FIA-HPLC system with zone sampling the LOD for developed method was evaluated as 15 ng/L, which indicates the possibility of using it for analysis of environmental samples.

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