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Fertil Steril. 2011 Jul;96(1):19-23.e2. doi: 10.1016/j.fertnstert.2011.04.048. Epub 2011 May 14.

Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients.

Author information

1
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, People's Republic of China.

Abstract

OBJECTIVE:

To determine whether the expression of DJ-1 protein, whose levels in spermatozoa have been reported to be highly correlated with male infertility caused by toxicants, is changed in spermatozoa of Chinese asthenozoospermia patients.

DESIGN:

DJ-1 measurement by Western blotting, quantitive ELISA, and isoelectric-focusing electrophoresis (IFE) combined with immunoblotting.

SETTING:

Academic medical center and research laboratories.

PATIENT(S):

Asthenozoospermia patients (n = 113), including mild asthenozoospermia patients (n = 70) and moderate asthenozoospermia patients (n = 43), and age-matched control subjects (n = 58).

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

DJ-1 in spermatozoa was determined by Western blotting and ELISA, the isoelectric point (pI) of DJ-1 by IFE combined with immunoblotting, and sperm superoxide dismutase (SOD) activity by an assay kit.

RESULT(S):

The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in asthenozoospermia patients.

CONCLUSION(S):

DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis.

[Indexed for MEDLINE]

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