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Traffic. 2011 Sep;12(9):1139-47. doi: 10.1111/j.1600-0854.2011.01221.x. Epub 2011 Jun 15.

Generic sorting of raft lipids into secretory vesicles in yeast.

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1
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, Dresden 01307, Germany.

Abstract

Previous work has showed that ergosterol and sphingolipids become sorted to secretory vesicles immunoisolated using a chimeric, artificial raft membrane protein as bait. In this study, we have extended this analysis to three populations of secretory vesicles isolated using natural yeast plasma membrane (PM) proteins: Pma1p, Mid2p and Gap1*p as baits. We compared the lipidomes of the immunoisolated vesicles with each other and with the lipidomes of the donor compartment, the trans-Golgi network, and the acceptor compartment, the PM, using a quantitative mass spectrometry approach that provided a complete lipid overview of the yeast late secretory pathway. We could show that vesicles captured with different baits carry the same cargo and have almost identical lipid compositions; being highly enriched in ergosterol and sphingolipids. This finding indicates that lipid raft sorting is a generic feature of vesicles carrying PM cargo and suggests a common lipid-based mechanism for their formation.

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