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Nat Struct Mol Biol. 2011 Jun;18(6):732-4. doi: 10.1038/nsmb.2052. Epub 2011 May 15.

Implications of molecular heterogeneity for the cooperativity of biological macromolecules.

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1
Department of Biochemistry, Stanford University, Stanford, California, USA.

Abstract

Cooperativity, a universal property of biological macromolecules, is typically characterized by a Hill slope, which can provide fundamental information about binding sites and interactions. We demonstrate, through simulations and single-molecule FRET (smFRET) experiments, that molecular heterogeneity lowers bulk cooperativity from the intrinsic value for the individual molecules. As heterogeneity is common in smFRET experiments, appreciation of its influence on fundamental measures of cooperativity is critical for deriving accurate molecular models.

PMID:
21572445
PMCID:
PMC3109240
DOI:
10.1038/nsmb.2052
[Indexed for MEDLINE]
Free PMC Article
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