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Macromol Rapid Commun. 2010 Sep 1;31(17):1509-15. doi: 10.1002/marc.201000337. Epub 2010 Jul 27.

Degradation of Hyper-Branched Poly(ethylenimine)-graft-poly(caprolactone)-block-monomethoxyl-poly(ethylene glycol) as a Potential Gene Delivery Vector.

Author information

1
Department of Pharmaceutics and Biopharmacy, Philipps-University of Marburg, Ketzerbach 63, 35037 Marburg, Germany.

Abstract

Water-soluble hyper-branched poly(ethylenimine)-graft-poly(caprolactone)-block-monomethoxyl-poly(ethylene glycol) cationic copolymers (hy-PEI-PCL-mPEG) have shown potential for gene delivery. The degradation behavior of this type of copolymer is studied. Effects of the molecular weight of the hyper-branched PEI (hy-PEI), and the length of the mPEG and PCL blocks are investigated. The degradation is mainly monitored by the changes of molecular weight by GPC-MALLS. The molecular weight changes of the copolymers in different aqueous solutions have been characterized as a function of incubation time at 37°C from 0 to 45 d. The micelle size and pH value have also been recorded accordingly to monitor the degradation in water. The investigation reveals that copolymers containing hy-PEI2k degrade faster than others containing hy-PEI10k and hy-PEI25k based on the same mPEG-PCL block, while copolymers those contain mPEG5k degrade faster than those containing mPEG2k and mPEG0.55k based on the same PCL segments and hy-PEI. An inhibitory effect of acidic conditions and acceleration in basic media have been found for degradation in different buffers. A higher ionic strength of the buffer accelerated the degradation. The molecular weight of copolymer is reduced to 95% within one day with 10 mg·mL(-1) of lipase while 70% remains with 5 mg·mL(-1) of lipase. These investigations suggest that the degradation of hy-PEI-PCL-mPEG can be designed and the degradation period is reasonable for gene-transfer in vivo.

PMID:
21567559
DOI:
10.1002/marc.201000337

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