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Mol Ecol Resour. 2009 Jan;9(1):281-4. doi: 10.1111/j.1755-0998.2008.02452.x. Epub 2008 Oct 30.

Characterization of polymorphic microsatellite loci in the endangered Miho spine loach (Iksookimia choii) and cross-species amplification within the Cobitidae family.

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Department of Marine Biotechnology, Soonchunhyang University, Asan 336-745, Korea Biotechnology Research Institute, National Fisheries Research and Development Institute, Busan 619-902, South Korea.


The Miho spine loach (Iksookimia choii) is an endangered freshwater fish endemic to Korea. To aid a conservation programme for this species, 20 polymorphic microsatellite loci were isolated and characterized in 67 individuals from three populations. The number of alleles per locus ranged from 8 to 33. Within populations, the observed and expected heterozygosities ranged from 0.59 to 1.00 and from 0.56 to 0.96, respectively, indicating its usefulness in population genetics studies. Cross-species amplification of the loci was tested in six species of the family Cobitidae, and 18 loci were amplified in two or more related species.

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