Aim: To study the anti-tumor mechanisms of ascidiacea analog SC002.
Methods: After treated the tumor cells with ascidiacea analog SC002, the changes of a serials of tumor associated cell signal molecules, including VEGF (vascular endothelial growth factor), P53, P21 and Bcl-2 et al, were detected by Western blot; further more the expression of serum VEGF in tumor-bearing mice and the activity of Caspase-3, 8, 9 in tumor tissue were investigated by ELISA; at last.
Results: Ascidiacea analog SC002 can obviously induce the expression of P53, while decreasing the expression of Bcl-2 and VEGF, but have on effect on p21 in tumor tissue; ELISA shown that SC002 can also reduce the concentration of serum VEGF in tumor bearing mice, but did not affected the activity of Caspase-3, 8 and 9.
Conclusion: SC002 have the anti-tumor function through promoting the expression of tumor suppressor gene p53, supress the expression of Bcl-2 and VEGF. This founding will provided an important base theory for it's further use as a novel anti-tumor drugs.