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PLoS One. 2011 Apr 29;6(4):e18963. doi: 10.1371/journal.pone.0018963.

Microscopic optical projection tomography in vivo.

Author information

1
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Crete, Greece.

Abstract

We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms.

PMID:
21559481
PMCID:
PMC3084718
DOI:
10.1371/journal.pone.0018963
[Indexed for MEDLINE]
Free PMC Article

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