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FEBS Lett. 1990 Feb 26;261(2):365-8.

Ubiquitinated protein conjugates are specifically enriched in the lysosomal system of fibroblasts.

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1
Department of General Zoology, ELTE University, Budapest, Hungary.

Abstract

Ubiquitin-protein conjugates are found by immunogold electron microscopy to be enriched (12-fold) in the lysosomal compartment of 3T3-L1 fibroblasts. Treatment of fibroblasts with the cysteine protease inhibitor E-64 leads to an expansion of the lysosomal compartment and as a result an increase in the cellular content of ubiquitin-protein conjugates. There is no change in the specific enrichment of ubiquitin-protein conjugates in the lysosomal compartment following E-64 treatment. The results suggest that some ubiquitin-protein conjugates may normally be degraded lysosomally following sequestration by microautophagy and imply that protein ubiquitination may be one of the signals for protein uptake into lysosomes.

PMID:
2155827
DOI:
10.1016/0014-5793(90)80593-8
[Indexed for MEDLINE]
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