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Nat Immunol. 2011 Jun;12(6):527-35. doi: 10.1038/ni.2036. Epub 2011 May 8.

Genetic analysis of basophil function in vivo.

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1
Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA.

Erratum in

  • Nat Immunol. 2011 Jun;12(6):3 p following 535.

Abstract

Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.

PMID:
21552267
PMCID:
PMC3271435
DOI:
10.1038/ni.2036
[Indexed for MEDLINE]
Free PMC Article

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