Format

Send to

Choose Destination
EMBO Rep. 2011 Jun;12(6):542-8. doi: 10.1038/embor.2011.72. Epub 2011 May 6.

Role of the import motor in insertion of transmembrane segments by the mitochondrial TIM23 complex.

Author information

1
Institute for Physiological Chemistry and Centre for Integrated Protein Science, Ludwig-Maximilians University, Butenandtstrasse 5, 81377 Munich, Germany.

Abstract

The TIM23 complex mediates translocation of proteins across, and their lateral insertion into, the mitochondrial inner membrane. Translocation of proteins requires both the membrane-embedded core of the complex and its ATP-dependent import motor. Insertion of some proteins, however, occurs in the absence of ATP, questioning the need for the import motor during lateral insertion. We show here that the import motor associates with laterally inserted proteins even when its ATPase activity is not required. Furthermore, our results suggest a role for the import motor in lateral insertion. Thus, the import motor is involved in ATP-dependent translocation and ATP-independent lateral insertion.

PMID:
21546912
PMCID:
PMC3128279
DOI:
10.1038/embor.2011.72
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center