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Dev Biol. 2011 Jul 1;355(1):77-88. doi: 10.1016/j.ydbio.2011.04.013. Epub 2011 Apr 22.

Essential roles of snap-29 in C. elegans.

Author information

1
Research Center for Functional Cellulomics, School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.

Abstract

SNARE domain proteins are key molecules mediating intracellular fusion events. SNAP25 family proteins are unique target-SNAREs possessing two SNARE domains. Here we report the genetic, molecular, and cell biological characterization of C. elegans SNAP-29. We found that snap-29 is an essential gene required throughout the life-cycle. Depletion of snap-29 by RNAi in adults results in sterility associated with endomitotic oocytes and pre-meiotic maturation of the oocytes. Many of the embryos that are produced are multinucleated, indicating a defect in embryonic cytokinesis. A profound defect in secretion by oocytes and early embryos in animals lacking SNAP-29 appears to be the underlying defect connecting these phenotypes. Further analysis revealed defects in basolateral and apical secretion by intestinal epithelial cells in animals lacking SNAP-29, indicating a broad requirement for this protein in the secretory pathway. A SNAP-29-GFP fusion protein was enriched on recycling endosomes, and loss of SNAP-29 disrupted recycling endosome morphology. Taken together these results suggest a requirement for SNAP-29 in the fusion of post-Golgi vesicles with the recycling endosome for cargo to reach the cell surface.

PMID:
21545795
PMCID:
PMC3118655
DOI:
10.1016/j.ydbio.2011.04.013
[Indexed for MEDLINE]
Free PMC Article

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