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Angew Chem Int Ed Engl. 2011 May 23;50(22):5024-32. doi: 10.1002/anie.201008267. Epub 2011 Apr 27.

Making and breaking peptide bonds: protein engineering using sortase.

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Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.


Sortases are a class of bacterial enzymes that possess transpeptidase activity. It is their ability to site-specifically break a peptide bond and then reform a new bond with an incoming nucleophile that makes sortase an attractive tool for protein engineering. This technique has been adopted for a range of applications, from chemistry-based to cell biology and technology. In this Minireview we provide a brief overview of the biology of sortase enzymes and current applications in protein engineering. We identify areas that lend themselves to further innovation and that suggest new applications.

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