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Biosens Bioelectron. 2011 Jun 15;26(10):4139-45. doi: 10.1016/j.bios.2011.04.009. Epub 2011 Apr 13.

Urea potentiometric enzymatic biosensor based on charged biopolymers and electrodeposited polyaniline.

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Institut UTINAM, UMR CNRS 6213, Equipe Matériaux et Surfaces Structurés, Université de Franche-Comté, 16 route de Gray, 25030 Besançon Cedex, France.


A potentiometric biosensor based on urease was developed for the quantitative determination of urea concentration in aqueous solutions for biomedical applications. The urease was either physisorbed onto an electrodeposited polyaniline film (PANI), or immobilized on a layer-by-layer film (LbL) assembled over the PANI film, that was obtained by the alternate deposition of charged polysaccharides (carboxymethylpullulan (CMP) and chitosan (CHI)). In the latter case, the urease (Urs) enzyme was either physically adsorbed or covalently grafted to the LbL film using carbodiimide coupling reaction. Potentiometric responses of the enzymatic biosensors were measured as a function of the urea concentration in aqueous solutions (from 10(-6) to 10(-1) mol L(-1) urea). Very high sensitivity and short response time were observed for the present biosensor. Moreover, a stability study showed a higher stability over time for the potentiometric response of the sensor with the enzyme-grafted LbL film, testifying for the protective nature of the polysaccharide coating and the interest of covalent grafting.

[Indexed for MEDLINE]

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