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Gend Med. 2011 Apr;8(2):126-38. doi: 10.1016/j.genm.2010.11.014.

The Sry3 Y chromosome locus elevates blood pressure and renin-angiotensin system indexes.

Author information

1
Department of Biology, University of Akron, Akron, Ohio 44325-3908, USA. ely1@uakron.edu

Abstract

BACKGROUND:

Sex-determining region Y (Sry) is a transcription factor. Our research group has shown that there are multiple copies of Sry in Wistar-Kyoto (WKY) and spontaneous hypertensive (SHR) rats, and that they have novel functions separate from testes determination.

OBJECTIVE:

We hypothesized that exogenously delivered Sry3 to the normotensive WKY male kidney would activate the renin-angiotensin system (RAS) and raise blood pressure (BP), based on previous in vitro studies.

METHODS:

Sry3 or control vector was electroporated to the left kidney of male WKY rats and the following measurements were taken: BP by telemetry, renin-angiotensin measures by radioimmunoassay, plasma and tissue catecholamines by HPLC with electrochemical detection, sodium by flame photometry, and inulin by ELISA.

RESULTS:

Sry3 increased BP 10 to 20 mm Hg compared with controls (P < 0.01) and produced a significant 40% decrease in urine sodium compared with controls (P < 0.05). Sry3 increased renal angiotensin II and plasma renin activity by >100% compared with controls (P < 0.01 and P < 0.05, respectively).

CONCLUSION:

The findings presented here confirm and extend the argument for Sry3 as one of the genes responsible for the SHR hypertensive Y chromosome phenotype and are consistent with increased tissue RAS activity due to Sry3 and increased sodium reabsorption.

PMID:
21536231
PMCID:
PMC3087190
DOI:
10.1016/j.genm.2010.11.014
[Indexed for MEDLINE]
Free PMC Article
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