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PLoS One. 2011 Apr 13;6(4):e18679. doi: 10.1371/journal.pone.0018679.

Porphyromonas gingivalis participates in pathogenesis of human abdominal aortic aneurysm by neutrophil activation. Proof of concept in rats.

Author information

1
Institut National de la Santé et de la Recherche Médicale U698, Paris, France.

Abstract

BACKGROUND:

Abdominal Aortic Aneurysms (AAAs) represent a particular form of atherothrombosis where neutrophil proteolytic activity plays a major role. We postulated that neutrophil recruitment and activation participating in AAA growth may originate in part from repeated episodes of periodontal bacteremia.

METHODS AND FINDINGS:

Our results show that neutrophil activation in human AAA was associated with Neutrophil Extracellular Trap (NET) formation in the IntraLuminal Thrombus, leading to the release of cell-free DNA. Human AAA samples were shown to contain bacterial DNA with high frequency (11/16), and in particular that of Porphyromonas gingivalis (Pg), the most prevalent pathogen involved in chronic periodontitis, a common form of periodontal disease. Both DNA reflecting the presence of NETs and antibodies to Pg were found to be increased in plasma of patients with AAA. Using a rat model of AAA, we demonstrated that repeated injection of Pg fostered aneurysm development, associated with pathological characteristics similar to those observed in humans, such as the persistence of a neutrophil-rich luminal thrombus, not observed in saline-injected rats in which a healing process was observed.

CONCLUSIONS:

Thus, the control of periodontal disease may represent a therapeutic target to limit human AAA progression.

PMID:
21533243
PMCID:
PMC3076426
DOI:
10.1371/journal.pone.0018679
[Indexed for MEDLINE]
Free PMC Article

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