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Nat Methods. 2011 May;8(5):393-9. doi: 10.1038/nmeth.1596. Epub 2011 Apr 28.

Two-photon absorption properties of fluorescent proteins.

Author information

1
Department of Physics, Montana State University, Bozeman, Montana, USA. drobizhev@physics.montana.edu

Abstract

Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right fluorescent protein and excitation wavelength for two-photon applications.

PMID:
21527931
PMCID:
PMC4772972
DOI:
10.1038/nmeth.1596
[Indexed for MEDLINE]
Free PMC Article
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