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J Neurosci. 2011 Apr 27;31(17):6311-6. doi: 10.1523/JNEUROSCI.5553-10.2011.

Molecular determinants controlling NMDA receptor synaptic incorporation.

Author information

1
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195-7290, USA.

Abstract

Synaptic incorporation of NMDA receptors (NMDARs) is regulated by GluN2 subunits with different rules controlling GluN2A- and GluN2B-containing receptors; whereas GluN2B-containing receptors are constitutively incorporated into synapses, GluN2A incorporation is activity-dependent. We expressed electrophysiologically tagged NMDARs in rat hippocampal slices to identify the molecular determinants controlling the mode of synaptic incorporation of NMDARs. Expressing chimeric GluN2 subunits, we identified a putative N-glycosylation site present in GluN2B, but not in GluN2A, as necessary and sufficient to drive NMDARs into synapses in an activity-independent manner. This suggests a novel mechanism for regulating activity-driven changes and trafficking of NMDARs to the synapse.

PMID:
21525271
PMCID:
PMC3092540
DOI:
10.1523/JNEUROSCI.5553-10.2011
[Indexed for MEDLINE]
Free PMC Article

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