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Methods Mol Biol. 2011;731:309-20. doi: 10.1007/978-1-61779-080-5_25.

Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay.

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1
Cancer Research UK Drug-DNA Interactions Research Group, UCL Cancer Institute, University College London, London, UK.

Abstract

The Single Cell Gel Electrophoresis (Comet) assay is a simple, versatile and sensitive method for measuring DNA damage in individual cells, allowing the determination of heterogeneity of response within a cell population. The basic alkaline technique described is for the determination of DNA strand break damage and its repair at a single cell level. Specific modifications to the method use a lower pH ('neutral' assay), or allow the measurement of DNA interstrand cross-links. It can be further adapted to, for example, study specific DNA repair mechanisms, be combined with fluorescent in situ hybridisation, or incorporate lesion specific enzymes.

PMID:
21516417
DOI:
10.1007/978-1-61779-080-5_25
[Indexed for MEDLINE]

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