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J Biomol Screen. 2011 Aug;16(7):775-85. doi: 10.1177/1087057111405851. Epub 2011 Apr 22.

Illustration of SSMD, z score, SSMD*, z* score, and t statistic for hit selection in RNAi high-throughput screens.

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Biometrics Research, Merck Research Laboratories, West Point, PA 19486, USA.


Hit selection is the ultimate goal in many high-throughput screens. Various analytic methods are available for this purpose. Some commonly used ones are z score, z* score, strictly standardized mean difference (SSMD), SSMD*, and t statistic. It is critical to know how to use them correctly because the misusage of them can readily produce misleading results. Here, the author presents basic concepts, elaborates their commonality and difference, describes some common misusage that people should avoid, and uses simulated simple examples to illustrate how to use them correctly.

[Indexed for MEDLINE]

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