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Biosci Biotechnol Biochem. 2011;75(4):646-9. Epub 2011 Apr 22.

Oviduct-specific enhanced green fluorescent protein expression in transgenic chickens.

Author information

1
Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Republic of Korea.

Abstract

In this study, we confirmed the ability of the 2-kb promoter fragment of the chicken ovalbumin gene to drive tissue-specific expression of a foreign EGFP gene in chickens. Recombinant lentiviruses containing the EGFP gene were injected into the subgerminal cavity of 539 freshly laid embryos (stage X). Subsequently the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Twenty-four chicks (G0) were hatched and screened for EGFP with PCR. Two chicks were identified as transgenic birds (G1), and these founders were mated with wild-type chickens to generate transgenic progeny. In the generated transgenic hens (G2), EGFP was expressed specifically in the tubular gland of the oviduct. These results show the potential of the chicken ovalbumin promoter for the production of biologically active proteins in egg white.

PMID:
21512248
DOI:
10.1271/bbb.100721
[Indexed for MEDLINE]
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