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Biosci Biotechnol Biochem. 2011;75(4):793-6. Epub 2011 Apr 22.

Characterization of recombinant β-amylases from Oryza sativa.

Author information

1
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan.

Abstract

Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5-6.0, but they exhibited inferior heat stability to soybean β-amylase.

PMID:
21512221
DOI:
10.1271/bbb.100872
[Indexed for MEDLINE]
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