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Biophys J. 2011 Apr 20;100(8):L37-9. doi: 10.1016/j.bpj.2011.03.007.

Active generation and propagation of Ca2+ signals within tunneling membrane nanotubes.

Author information

1
Department of Neurobiology and Behavior, University of California, Irvine, California, USA. ismith@uci.edu

Abstract

A new mechanism of cell-cell communication was recently proposed after the discovery of tunneling nanotubes (TNTs) between cells. TNTs are membrane protrusions with lengths of tens of microns and diameters of a few hundred nanometers that permit the exchange of membrane and cytoplasmic constituents between neighboring cells. TNTs have been reported to mediate intercellular Ca(2+) signaling; however, our simulations indicate that passive diffusion of Ca(2+) ions alone would be inadequate for efficient transmission between cells. Instead, we observed spontaneous and inositol trisphosphate (IP(3))-evoked Ca(2+) signals within TNTs between cultured mammalian cells, which sometimes remained localized and in other instances propagated as saltatory waves to evoke Ca(2+) signals in a connected cell. Consistent with this, immunostaining showed the presence of both endoplasmic reticulum and IP(3) receptors along the TNT. We propose that IP(3) receptors may actively propagate intercellular Ca(2+) signals along TNTs via Ca(2+)-induced Ca(2+) release, acting as amplification sites to overcome the limitations of passive diffusion in a chemical analog of electrical transmission of action potentials.

PMID:
21504718
PMCID:
PMC3077701
DOI:
10.1016/j.bpj.2011.03.007
[Indexed for MEDLINE]
Free PMC Article

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