Format

Send to

Choose Destination
Leuk Lymphoma. 2011 Jun;52 Suppl 2:11-3. doi: 10.3109/10428194.2011.570820. Epub 2011 Apr 19.

Diagnosis of hairy cell leukemia by flow cytometry.

Author information

1
Flow Cytometry Unit, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. stetler@mail.nih.gov

Abstract

Flow cytometric immunophenotpying (FCI) is a vital tool in the diagnosis of hairy cell leukemia (HCL). HCL has a distinctive immunophenotype based upon staining with antibodies to CD5 (negative), CD10 (negative), CD23 (negative), CD20 (abnormally bright), CD22 (abnormally bright), CD11c (abnormally bright), CD25 (abnormally bright), CD103 (positive), and CD123 (positive). Due to this unique immunophenotype, FCI can differentiate malignant hairy cells from normal B-cells and other lymphoproliferative disorders, especially the morphologically similar hairy cell leukemia variant and splenic lymphoma with villous lymphocytes. FCI is extremely sensitive in the detection of minimal disease and is useful in monitoring response to therapy.

PMID:
21504292
DOI:
10.3109/10428194.2011.570820
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center