Mariniflexile aquimaris sp. nov., isolated from seawater, and emended description of the genus Mariniflexile Nedashkovskaya et al. 2006

Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):539-544. doi: 10.1099/ijs.0.031492-0. Epub 2011 Apr 15.

Abstract

A Gram-staining-negative, non-flagellated, non-gliding and rod-shaped bacterial strain, designated HWR-17(T), was isolated from seawater of the Yellow Sea in Korea. Strain HWR-17(T) grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 2% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HWR-17(T) clustered with the two Mariniflexile species in the family Flavobacteriaceae, exhibiting 16S rRNA gene sequence similarity of 97.1-97.2% to their type strains and less than 95.7% sequence similarity to other members of the family Flavobacteriaceae. Strain HWR-17(T) contained MK-6 as the predominant menaquinone and iso-C(15:0) as the major fatty acid. The polar lipid profile of strain HWR-17(T) contained phosphatidylethanolamine, an unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain HWR-17(T) was 35.7 mol% and it exhibited 11 and 10% DNA-DNA relatedness, respectively, with Mariniflexile gromovii KCTC 12570(T) and Mariniflexile fucanivorans DSM 18792(T). The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain HWR-17(T) is distinguishable from the two recognized Mariniflexile species. On the basis of the data presented, strain HWR-17(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile aquimaris sp. nov. is proposed. The type strain is HWR-17(T) (=KCTC 23346(T) =CCUG 60529(T)). An emended description of the genus Mariniflexile is also proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / genetics
  • Flavobacteriaceae / isolation & purification*
  • Flavobacteriaceae / physiology
  • Glucosidases / analysis
  • Hydrogen-Ion Concentration
  • Korea
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phospholipids / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Temperature
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Sodium Chloride
  • Glucosidases

Associated data

  • GENBANK/HQ144198