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PLoS One. 2011 Apr 6;6(4):e18342. doi: 10.1371/journal.pone.0018342.

Protein-protein fusion catalyzed by sortase A.

Author information

1
Department of Physics, Harvard University, Cambridge, Massachusetts, United States of America.

Abstract

Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality--demonstrating the robust and facile nature of this reaction.

PMID:
21494692
PMCID:
PMC3071835
DOI:
10.1371/journal.pone.0018342
[Indexed for MEDLINE]
Free PMC Article
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